Ultimate Guide: Progesterone Testing for Dogs – 3 Proven Steps to Breed & How to Choose the Right Stud Dog

If you’re getting ready to breed your female dog, timing is crucial and is everything, The most accurate way to know if your female is ready is through a progesterone testing done through a veterinary clinic. Understanding your dog’s hormone levels can dramatically increase your chances of a successful, healthy litter whether you are planning a natural mating or artificial insemination.

One of the most common questions stud dog owners get asked is “When is the right time to bring my female?”

The very simple answer is : When progesterone levels indicate ovulation is near or just occurred.

Let’s break down what progesterone testing is, why it’s essential, and how to prepare for finding the right stud.

What Is Progesterone Testing in Dogs?

Progesterone testing is a blood test performed by your vet to measure your female dog’s progesterone hormone levels. This hormone rises as she nears ovulation making it the most reliable method for timing breeding.

Unlike visual signs (like vulva swelling or flagging), progesterone testing gives you scientific data on when your dog is most fertile. This is especially important if:

  • You’re traveling to meet a stud dog

  • You’re using artificial insemination (AI) or chilled/frozen semen

  • Your female has missed in previous cycles

The Ideal Progesterone Levels for Breeding

When it comes to timing breeding, progesterone testing helps take the guesswork out of knowing when your female is ready and also avoiding disappointments later. If your females progesterone level is below 1, she’s definitely not ready yet. Around 2 to 3, her body is getting close — that’s usually when the LH surge happens, which means ovulation is coming in a day or two.

Once levels hit about 5 to 8, ovulation is likely happening, and things are moving fast. The ideal time to breed is usually when levels reach 10 or higher. At that point, the eggs are mature and she’s most fertile.

Because every dog is a bit different, vets often test every couple of days during her heat cycle to find that perfect window. If you’re using a stud dog like Loki, sharing these results helps us plan the best timing for a successful mating, whether it’s natural or artificial insemination.

Preparing to Find a Stud Dog: What You Need in Place

Before reaching out to a stud dog owner, make sure you’re fully prepared. A well-prepared breeder = a smooth, successful mating experience.

1. Start Progesterone Testing Early

Begin testing around day 6–8 of her heat cycle. It can vary between dogs, so earlier is better than too late.

2. Know What You Want in a Stud

Consider the following:

  • Size compatibility (especially if breeding a toy or mini poodle)

  • Coat type, color, and furnishings

  • Temperament

  • Proven fertility

 3. Have Logistics Ready

  • Will it be natural mating, AI, or chilled/frozen semen?

  • Are you traveling or is semen being shipped?

  • Are payment terms clear?

  • What options is the stud owner offering for unsuccessful mating?

Why Progesterone Testing Helps You Get the Most From a Stud Dog

As a breeder progesterone testing is the most valuable thing for your breeding journey, It:

  • Increases your chance of a successful pregnancy

  • Minimizes wasted travel or missed matings

  • Makes the process smoother for everyone involve

We work closely with breeders who provide progesterone test results so we can plan the best possible mating date. Whether it’s for natural service or semen shipment, this timing makes all the difference.

Ready to Book a Poodle Stud Dog ?

If you’re progesterone testing and preparing for breeding, you’re in the perfect window to start talking to stud dog owners. We offer stud services to all Oodles breeders.  Our stud, Loki, is a proven sire with multiple successful litters, and his semen analysis shows excellent quality and motility, making him a reliable choice for your breeding program.


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